primary antibodies against nanog (Santa Cruz Biotechnology)
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Primary Antibodies Against Nanog, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+nanog/Nanog+Antibody/pm39040103-89-0-13
Average 96 stars, based on 754 article reviews
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1) Product Images from "A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency."
Article Title: A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency.
Journal: Frontiers in immunology
doi: 10.3389/fimmu.2024.1419748
Figure Legend Snippet: FIGURE 1 Molecular characterization of pYM-iPSCs. (A) Expression of pluripotency markers hTERT, LIN28, SOX2, DNMT3B, OCT3/4, NANOG and KLF4 transcripts measured by RT-PCR in a representative clone of pYM-iPSCs (C6); (B) Expression of OCT3/4, NANOG and DNMT3B pluripotency markers measured by immunofluorescence in the pYM C6 clone. DAPI, nuclear staining. Bar: 100mm; (C) Dot plot showing the expression of the pluripotency marker SSEA4 in the pYM C6 clone compared to the negative control (CTRL-). PE-CF594-A, conjugated secondary antibody; (D) Karyotype analysis demonstrating chromosomal integrity of the pYM C6 clone; (E) Immunostaining of markers representing the three embryonic germ layers, GATA4 (endoderm), bIII-tubulin (ectoderm) and MF20 (mesoderm) expressed in the in vitro differentiated C6 clone. Bar: 100 mm; (F) ZBTB24, CDCA7, CDC40 and OSTC transcripts level in control (C14 and C22) and pYM (C6 and B9) iPSC clones measured by qPCR. The relative expression compared to the expression of GAPDH is shown for each gene. qPCR data are presented as mean ± SD from independent triplicates. Statistical analyses were performed using a one-side two-sample Student’s t-test compared to control: (*) P-value < 0.05, (***) P-value < 0.001.
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunostaining, In Vitro, Control, Clone Assay
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