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primary antibodies against nanog  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology primary antibodies against nanog
    FIGURE 1 Molecular characterization of pYM-iPSCs. (A) Expression of pluripotency markers hTERT, LIN28, SOX2, DNMT3B, OCT3/4, <t>NANOG</t> and KLF4 transcripts measured by RT-PCR in a representative clone of pYM-iPSCs (C6); (B) Expression of OCT3/4, NANOG and DNMT3B pluripotency markers measured by immunofluorescence in the pYM C6 clone. DAPI, nuclear staining. Bar: 100mm; (C) Dot plot showing the expression of the pluripotency marker SSEA4 in the pYM C6 clone compared to the negative control (CTRL-). PE-CF594-A, conjugated secondary antibody; (D) Karyotype analysis demonstrating chromosomal integrity of the pYM C6 clone; (E) Immunostaining of markers representing the three embryonic germ layers, GATA4 (endoderm), bIII-tubulin (ectoderm) and MF20 (mesoderm) expressed in the in vitro differentiated C6 clone. Bar: 100 mm; (F) ZBTB24, CDCA7, CDC40 and OSTC transcripts level in control (C14 and C22) and pYM (C6 and B9) iPSC clones measured by qPCR. The relative expression compared to the expression of GAPDH is shown for each gene. qPCR data are presented as mean ± SD from independent triplicates. Statistical analyses were performed using a one-side two-sample Student’s t-test compared to control: (*) P-value < 0.05, (***) P-value < 0.001.
    Primary Antibodies Against Nanog, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency."

    Article Title: A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency.

    Journal: Frontiers in immunology

    doi: 10.3389/fimmu.2024.1419748

    FIGURE 1 Molecular characterization of pYM-iPSCs. (A) Expression of pluripotency markers hTERT, LIN28, SOX2, DNMT3B, OCT3/4, NANOG and KLF4 transcripts measured by RT-PCR in a representative clone of pYM-iPSCs (C6); (B) Expression of OCT3/4, NANOG and DNMT3B pluripotency markers measured by immunofluorescence in the pYM C6 clone. DAPI, nuclear staining. Bar: 100mm; (C) Dot plot showing the expression of the pluripotency marker SSEA4 in the pYM C6 clone compared to the negative control (CTRL-). PE-CF594-A, conjugated secondary antibody; (D) Karyotype analysis demonstrating chromosomal integrity of the pYM C6 clone; (E) Immunostaining of markers representing the three embryonic germ layers, GATA4 (endoderm), bIII-tubulin (ectoderm) and MF20 (mesoderm) expressed in the in vitro differentiated C6 clone. Bar: 100 mm; (F) ZBTB24, CDCA7, CDC40 and OSTC transcripts level in control (C14 and C22) and pYM (C6 and B9) iPSC clones measured by qPCR. The relative expression compared to the expression of GAPDH is shown for each gene. qPCR data are presented as mean ± SD from independent triplicates. Statistical analyses were performed using a one-side two-sample Student’s t-test compared to control: (*) P-value < 0.05, (***) P-value < 0.001.
    Figure Legend Snippet: FIGURE 1 Molecular characterization of pYM-iPSCs. (A) Expression of pluripotency markers hTERT, LIN28, SOX2, DNMT3B, OCT3/4, NANOG and KLF4 transcripts measured by RT-PCR in a representative clone of pYM-iPSCs (C6); (B) Expression of OCT3/4, NANOG and DNMT3B pluripotency markers measured by immunofluorescence in the pYM C6 clone. DAPI, nuclear staining. Bar: 100mm; (C) Dot plot showing the expression of the pluripotency marker SSEA4 in the pYM C6 clone compared to the negative control (CTRL-). PE-CF594-A, conjugated secondary antibody; (D) Karyotype analysis demonstrating chromosomal integrity of the pYM C6 clone; (E) Immunostaining of markers representing the three embryonic germ layers, GATA4 (endoderm), bIII-tubulin (ectoderm) and MF20 (mesoderm) expressed in the in vitro differentiated C6 clone. Bar: 100 mm; (F) ZBTB24, CDCA7, CDC40 and OSTC transcripts level in control (C14 and C22) and pYM (C6 and B9) iPSC clones measured by qPCR. The relative expression compared to the expression of GAPDH is shown for each gene. qPCR data are presented as mean ± SD from independent triplicates. Statistical analyses were performed using a one-side two-sample Student’s t-test compared to control: (*) P-value < 0.05, (***) P-value < 0.001.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunostaining, In Vitro, Control, Clone Assay

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    Article Title: Sequential growth factor exposure of human Ad-MSCs improves chondrogenic differentiation in an osteochondral biphasic implant.
    Article Snippet: Samples were then washed with PBS‐Tween 20 (0.01%) and incubated with anti‐mouse IgG secondary antibody conjugated with Alexa Fluor® 488 (cat. no. ab150113; 1:1,000) and rhodamine phalloidin (cat. no. ab235138; 1:60; all from Abcam) for 2 h at room temper‐ ature to stain actin filaments.

    Article Title: Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells
    Article Snippet: The cells were then incubated with a blocking solution containing primary antibodies against OCT4 (1:200; rabbit polyclonal, Santa Cruz Biotechnology, catalog number: sc-9081), NANOG (1:200; rabbit polyclonal, Santa Cruz Biotechnology, catalog number: sc-33759), or OCN (1:100; mouse monoclonal, Santa Cruz Biotechnology, catalog number: sc-74495) at 4 °C for 16 hours.

    Incubation:

    Article Title: A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency
    Article Snippet: .. Primary antibodies against NANOG (SCTB, sc-293121), DNMT3B (Diagenode, pAb-076-005), OCT3/4 (SCTB, sc-5279), GATA4 (SCBT, SC25310), βIII-tubulin (Sigma-Aldrich, T8660), MF20 (DSHB, RRID:AB_2147781) were incubated overnight at 4 °C. .. The cells were then incubated with secondary antibodies: AlexaFluor 594 conjugated goat anti-mouse IgG, AlexaFluor 594 conjugated donkey anti-rabbit IgG (ThermoFisher).

    Article Title: Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type
    Article Snippet: Images were taken using a microscope (Olympus CKX41, Tokyo, Japan). .. Immunofluorescence staining was performed to detect pluripotency markers. iPSCs of Alport syndrome patients and the healthy control were fixed with 4% paraformaldehyde, blocked with PBS containing 0.3% Triton X-100, and 10% BSA for 1 h. Primary antibodies against Nanog (SANTA CRUZ, Cat NO. sc-293121) and Oct-3/4 (SANTA CRUZ, Cat NO. sc-5279) in PBS containing 0.3% Triton X-100 and 1% BSA were then incubated with the fixed cells at 4°C overnight. .. After washing three times with PBS containing 0.1% Triton X-100 for 30 min, cells were incubated with secondary antibodies conjugated with Alexa Fluor 561 (Thermo Fisher Scientific, Cat NO. A-11062) at 4°C overnight.

    Article Title: Sequential growth factor exposure of human Ad-MSCs improves chondrogenic differentiation in an osteochondral biphasic implant
    Article Snippet: Cells were permeabilized with 0.05% Triton X-100 (cat. no. T8787; Sigma-Aldrich; Merck KGaA) for 5 min, then washed with PBS and blocked for non-specific sites by treatment with 0.5% bovine serum albumin (cat. no. A3294; Sigma-Aldrich; Merck KGaA) for 30 min at room temperature. .. Cells were incubated overnight at 4 ̊C with a 1:100 dilution of specific primary antibodies against NANOG (cat. no. sc-293121), OCT3/4 (cat. no. sc-5279) and SSEA4 (cat. no. sc-21704; all from Santa Cruz Biotechnology, Inc.) in a wet chamber. .. Samples were then washed with PBS-Tween 20 (0.01%) and incubated with anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 (cat. no. ab150113; 1:1,000) and rhodamine phalloidin (cat. no. ab235138; 1:60; all from Abcam) for 2 h at room temperature to stain actin filaments.

    Article Title: Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells
    Article Snippet: .. For immunouorescent staining, the cells were xed with 4% paraformaldehyde at 25 C for 10 minutes and blocked for 30 minutes in a buffer solution containing 3% bovine serum albumin (BSA) and 0.1% TritonTM X-100 at 25 C. The xed cells were incubated with primary antibodies against NANOG (1 : 200; rabbit polyclonal, Santa Cruz Biotechnology, catalog This journal is © The Royal Society of Chemistry 2014 Pu bl is he d on 0 4 Ju ly 2 01 4. .. D ow nl oa de d on 1 5/ 10 /2 01 4 23 :4 0: 56 number: sc-33759), OCT4 (1 : 200; rabbit polyclonal, Santa Cruz Biotechnology, catalog number: sc-9081) or OCN (1 : 100; mouse monoclonal, Abcam, catalog number: ab13420) in a blocking solution at 4 C for 16 hours.

    Article Title: Sequential growth factor exposure of human Ad-MSCs improves chondrogenic differentiation in an osteochondral biphasic implant.
    Article Snippet: Cells were permeabilized with 0.05% Triton X‐100 (cat. no. T8787; Sigma‐Aldrich; Merck KGaA) for 5 min, then washed with PBS and blocked for non‐specific sites by treatment with 0.5% bovine serum albumin (cat. no. A3294; Sigma‐Aldrich; Merck KGaA) for 30 min at room temperature. .. Cells were incubated overnight at 4 ̊C with a 1:100 dilution of specific primary antibodies against NANOG (cat. no. sc‐293121), OCT3/4 (cat. no. sc‐5279) and SSEA4 (cat. no. sc‐21704; all from Santa Cruz Biotechnology, Inc.) in a wet chamber. .. Samples were then washed with PBS‐Tween 20 (0.01%) and incubated with anti‐mouse IgG secondary antibody conjugated with Alexa Fluor® 488 (cat. no. ab150113; 1:1,000) and rhodamine phalloidin (cat. no. ab235138; 1:60; all from Abcam) for 2 h at room temper‐ ature to stain actin filaments.

    Article Title: A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency.
    Article Snippet: .. Primary antibodies against NANOG (SCTB, sc-293121), DNMT3B (Diagenode, pAb-076-005), OCT3/4 (SCTB, sc-5279), GATA4 (SCBT, SC25310), bIII-tubulin (Sigma-Aldrich, T8660), MF20 (DSHB, RRID: AB_2147781) were incubated overnight at 4° C. The cells were then incubated with secondary antibodies: AlexaFluor 594 conjugated goat anti-mouse IgG, AlexaFluor 594 Frontiers in Immunology 03 conjugated donkey anti-rabbit IgG (ThermoFisher). ..

    Immunofluorescence:

    Article Title: Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type
    Article Snippet: Images were taken using a microscope (Olympus CKX41, Tokyo, Japan). .. Immunofluorescence staining was performed to detect pluripotency markers. iPSCs of Alport syndrome patients and the healthy control were fixed with 4% paraformaldehyde, blocked with PBS containing 0.3% Triton X-100, and 10% BSA for 1 h. Primary antibodies against Nanog (SANTA CRUZ, Cat NO. sc-293121) and Oct-3/4 (SANTA CRUZ, Cat NO. sc-5279) in PBS containing 0.3% Triton X-100 and 1% BSA were then incubated with the fixed cells at 4°C overnight. .. After washing three times with PBS containing 0.1% Triton X-100 for 30 min, cells were incubated with secondary antibodies conjugated with Alexa Fluor 561 (Thermo Fisher Scientific, Cat NO. A-11062) at 4°C overnight.

    Staining:

    Article Title: Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type
    Article Snippet: Images were taken using a microscope (Olympus CKX41, Tokyo, Japan). .. Immunofluorescence staining was performed to detect pluripotency markers. iPSCs of Alport syndrome patients and the healthy control were fixed with 4% paraformaldehyde, blocked with PBS containing 0.3% Triton X-100, and 10% BSA for 1 h. Primary antibodies against Nanog (SANTA CRUZ, Cat NO. sc-293121) and Oct-3/4 (SANTA CRUZ, Cat NO. sc-5279) in PBS containing 0.3% Triton X-100 and 1% BSA were then incubated with the fixed cells at 4°C overnight. .. After washing three times with PBS containing 0.1% Triton X-100 for 30 min, cells were incubated with secondary antibodies conjugated with Alexa Fluor 561 (Thermo Fisher Scientific, Cat NO. A-11062) at 4°C overnight.

    Article Title: Biomineralized matrix-assisted osteogenic differentiation of human embryonic stem cells
    Article Snippet: .. For immunouorescent staining, the cells were xed with 4% paraformaldehyde at 25 C for 10 minutes and blocked for 30 minutes in a buffer solution containing 3% bovine serum albumin (BSA) and 0.1% TritonTM X-100 at 25 C. The xed cells were incubated with primary antibodies against NANOG (1 : 200; rabbit polyclonal, Santa Cruz Biotechnology, catalog This journal is © The Royal Society of Chemistry 2014 Pu bl is he d on 0 4 Ju ly 2 01 4. .. D ow nl oa de d on 1 5/ 10 /2 01 4 23 :4 0: 56 number: sc-33759), OCT4 (1 : 200; rabbit polyclonal, Santa Cruz Biotechnology, catalog number: sc-9081) or OCN (1 : 100; mouse monoclonal, Abcam, catalog number: ab13420) in a blocking solution at 4 C for 16 hours.

    Control:

    Article Title: Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type
    Article Snippet: Images were taken using a microscope (Olympus CKX41, Tokyo, Japan). .. Immunofluorescence staining was performed to detect pluripotency markers. iPSCs of Alport syndrome patients and the healthy control were fixed with 4% paraformaldehyde, blocked with PBS containing 0.3% Triton X-100, and 10% BSA for 1 h. Primary antibodies against Nanog (SANTA CRUZ, Cat NO. sc-293121) and Oct-3/4 (SANTA CRUZ, Cat NO. sc-5279) in PBS containing 0.3% Triton X-100 and 1% BSA were then incubated with the fixed cells at 4°C overnight. .. After washing three times with PBS containing 0.1% Triton X-100 for 30 min, cells were incubated with secondary antibodies conjugated with Alexa Fluor 561 (Thermo Fisher Scientific, Cat NO. A-11062) at 4°C overnight.



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    Image Search Results


    B7H6 expression is maintained in motoneurons differentiated from iPS-derived neural progenitor cells. (A) B7H6 (NCR3LG1) transcription was assessed by qPCR analysis using cDNA from the indicated cells, displaying relative expression using GNB2LI as housekeeping control. The tumor cell lines HCT15 and MDA.MB.231 have been included as B7H6+ and B7H6− controls, respectively. The results are normalized and represent 3 or more independent triplicate experiments. Neurons were generated from iPS-derived NPCs, and their phenotype was documented by (B) phase contrast microscopy demonstrating dendritic morphology, and (C) qPCR analysis for the neuronal markers NeuN (RBFOX3), MAP-2 (MAP2), class III β-tubulin (TuJ1; gene TUBB3), and synaptotagmin (SYT1), as indicated. NPC phenotype was defined by downregulation of the pluripotency marker Oct4 (POU5F1) and concomitant upregulation of nestin (NES). Expression was calculated using β-actin as an internal control. Error bars indicate SD based on 1 triplicate experiment.

    Journal: Stem Cells

    Article Title: NK-cell cytotoxicity toward pluripotent stem cells and their neural progeny: impacts of activating and inhibitory receptors and KIR/HLA mismatch

    doi: 10.1093/stmcls/sxae083

    Figure Lengend Snippet: B7H6 expression is maintained in motoneurons differentiated from iPS-derived neural progenitor cells. (A) B7H6 (NCR3LG1) transcription was assessed by qPCR analysis using cDNA from the indicated cells, displaying relative expression using GNB2LI as housekeeping control. The tumor cell lines HCT15 and MDA.MB.231 have been included as B7H6+ and B7H6− controls, respectively. The results are normalized and represent 3 or more independent triplicate experiments. Neurons were generated from iPS-derived NPCs, and their phenotype was documented by (B) phase contrast microscopy demonstrating dendritic morphology, and (C) qPCR analysis for the neuronal markers NeuN (RBFOX3), MAP-2 (MAP2), class III β-tubulin (TuJ1; gene TUBB3), and synaptotagmin (SYT1), as indicated. NPC phenotype was defined by downregulation of the pluripotency marker Oct4 (POU5F1) and concomitant upregulation of nestin (NES). Expression was calculated using β-actin as an internal control. Error bars indicate SD based on 1 triplicate experiment.

    Article Snippet: Following permeabilization in 0.1% Triton, the cells were stained with primary antibodies against the pluripotency markers Nanog (clone 7F7.1; 1:400; Millipore), Oct4, and SOX2 (cat. no. 090023 and 090024, respectively; 1:100; StemGent) overnight at 4 °C, washed, incubated for 1 hour at room temperature with Cy2-conjugated or Cy3-conjugated secondary antibodies (1:400; Jackson ImmunoResearch) followed by counterstaining of nuclei with DAPI.

    Techniques: Expressing, Derivative Assay, Control, Generated, Microscopy, Marker

    Immunofluorescence and qPCR characterization of BatiPSC. To identify the cell type most closely resembling iPSCs, we conducted OCT4 (scale bar = 250 µm) and nanog (scale bar = 75 µm) immunofluorescence staining (A) and RT-qPCR (B). The findings demonstrated strong expression of pluripotent stem cell markers, particularly in colonies with a dome-shaped structure. Asterisks were used to indicate statistical significance between the groups (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: International Journal of Veterinary Science and Medicine

    Article Title: Generation of induced pluripotent stem cells from the Asian bats

    doi: 10.1080/23144599.2024.2384835

    Figure Lengend Snippet: Immunofluorescence and qPCR characterization of BatiPSC. To identify the cell type most closely resembling iPSCs, we conducted OCT4 (scale bar = 250 µm) and nanog (scale bar = 75 µm) immunofluorescence staining (A) and RT-qPCR (B). The findings demonstrated strong expression of pluripotent stem cell markers, particularly in colonies with a dome-shaped structure. Asterisks were used to indicate statistical significance between the groups (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: The cells were subsequently incubated with primary mouse antibodies against Oct4 (P0082-200UL, Sigma-Aldrich), and primary rabbit antibodies against Nanog (RCAB004P-F, ReproCELL, Beltsville, MD, USA) at 4°C overnight.

    Techniques: Immunofluorescence, Staining, Quantitative RT-PCR, Expressing

    FIGURE 1 Molecular characterization of pYM-iPSCs. (A) Expression of pluripotency markers hTERT, LIN28, SOX2, DNMT3B, OCT3/4, NANOG and KLF4 transcripts measured by RT-PCR in a representative clone of pYM-iPSCs (C6); (B) Expression of OCT3/4, NANOG and DNMT3B pluripotency markers measured by immunofluorescence in the pYM C6 clone. DAPI, nuclear staining. Bar: 100mm; (C) Dot plot showing the expression of the pluripotency marker SSEA4 in the pYM C6 clone compared to the negative control (CTRL-). PE-CF594-A, conjugated secondary antibody; (D) Karyotype analysis demonstrating chromosomal integrity of the pYM C6 clone; (E) Immunostaining of markers representing the three embryonic germ layers, GATA4 (endoderm), bIII-tubulin (ectoderm) and MF20 (mesoderm) expressed in the in vitro differentiated C6 clone. Bar: 100 mm; (F) ZBTB24, CDCA7, CDC40 and OSTC transcripts level in control (C14 and C22) and pYM (C6 and B9) iPSC clones measured by qPCR. The relative expression compared to the expression of GAPDH is shown for each gene. qPCR data are presented as mean ± SD from independent triplicates. Statistical analyses were performed using a one-side two-sample Student’s t-test compared to control: (*) P-value < 0.05, (***) P-value < 0.001.

    Journal: Frontiers in immunology

    Article Title: A novel iPSC-based model of ICF syndrome subtype 2 recapitulates the molecular phenotype of ZBTB24 deficiency.

    doi: 10.3389/fimmu.2024.1419748

    Figure Lengend Snippet: FIGURE 1 Molecular characterization of pYM-iPSCs. (A) Expression of pluripotency markers hTERT, LIN28, SOX2, DNMT3B, OCT3/4, NANOG and KLF4 transcripts measured by RT-PCR in a representative clone of pYM-iPSCs (C6); (B) Expression of OCT3/4, NANOG and DNMT3B pluripotency markers measured by immunofluorescence in the pYM C6 clone. DAPI, nuclear staining. Bar: 100mm; (C) Dot plot showing the expression of the pluripotency marker SSEA4 in the pYM C6 clone compared to the negative control (CTRL-). PE-CF594-A, conjugated secondary antibody; (D) Karyotype analysis demonstrating chromosomal integrity of the pYM C6 clone; (E) Immunostaining of markers representing the three embryonic germ layers, GATA4 (endoderm), bIII-tubulin (ectoderm) and MF20 (mesoderm) expressed in the in vitro differentiated C6 clone. Bar: 100 mm; (F) ZBTB24, CDCA7, CDC40 and OSTC transcripts level in control (C14 and C22) and pYM (C6 and B9) iPSC clones measured by qPCR. The relative expression compared to the expression of GAPDH is shown for each gene. qPCR data are presented as mean ± SD from independent triplicates. Statistical analyses were performed using a one-side two-sample Student’s t-test compared to control: (*) P-value < 0.05, (***) P-value < 0.001.

    Article Snippet: Primary antibodies against NANOG (SCTB, sc-293121), DNMT3B (Diagenode, pAb-076-005), OCT3/4 (SCTB, sc-5279), GATA4 (SCBT, SC25310), bIII-tubulin (Sigma-Aldrich, T8660), MF20 (DSHB, RRID: AB_2147781) were incubated overnight at 4° C. The cells were then incubated with secondary antibodies: AlexaFluor 594 conjugated goat anti-mouse IgG, AlexaFluor 594 Frontiers in Immunology 03 conjugated donkey anti-rabbit IgG (ThermoFisher).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunostaining, In Vitro, Control, Clone Assay